Development of a high-throughput method for detection of the behavior of plant epigenome.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660008
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Satoh Soichirou 京都府立大学, 生命環境科学研究科(系), 研究員 (00399809)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 転写 / エピジェネティクス / プロモーター / 次世代シーケンサー / クロマチン / 進化 / ジーントラップ / ゲノム

Outline of Final Research Achievements

In the plant genome, de novo origination of genes or transcribed regions is occasionally caused by the integration of foreign DNA fragments. To clarify this mechanism, we tried to develop new comprehensive method for the detection of epigenetic states of the reporter genes that were randomly integrated into the plant genome. As a result, we succeeded in the development of a prototypic experimental system, and found that majority of foreign genes acquired transcriptional competence not by the intrinsic promoters in the upstream region of foreign genes but by the novel transcriptional activation mechanism.

Free Research Field

植物分子生物学