Stidies on aluminum tolerance of hydrangea for developping a new strategy against acidic soils

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660014
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Crop production science
• Research Institution: Nagoya University
• Principal Investigator: Yoshida Kumi 名古屋大学, 情報科学研究科, 教授 (90210690)
• Co-Investigator(Renkei-kenkyūsha): TURO Masato 名城大学, 農学部, 准教授 (40410774)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アジサイ / 酸性土壌耐性 / アルミニウム毒性

Outline of Final Research Achievements

Hydrangea macrohpylla accumulates Al3+ in the sepal vacuoles, which is responsible for blue coloration. This phenomenon would be involved in the Al tolerance of hydrangea in acidic soils. To clarify the mechanism of Al-tolerance, blue-cultivars of hydrangea were grown on a acidic and neutral soils from cuttage stage to fully blooming, then, the composition of organic components, Al content and expression level of genes involving in Al-tolerance were analyzed.
Expression level of HmVALT and Al content in leaf of +Al was higher than that of-Al. It is suggested that expression of genes involving Al-tolerance might be induced by Al-treatment at the early stage. The overexpression of HmVALT in Arabidopsis thaliana conferred Al tolerance, therefore, we cultivated A. thaliana with/without Al until the mature stage, then, the Al content and gene expression levels were analyzed. The growth of wild type (Col-0) was defected while the HmVALT overexpressed plant was grown without any damage.

Free Research Field

生物有機科学