2015 Fiscal Year Final Research Report
Identification of genes involving in mesophyll and bundle sheath cell-specific functions in gramineous plants by using a rapid cell separation technique
| Project/Area Number |
26660015
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
OI Takao 名古屋大学, 大学院生命農学研究科, 助教 (60752219)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | C4植物 / 葉緑体 / 環境ストレス / 葉肉細胞 / 維管束鞘細胞 / トウモロコシ / 遺伝子発現 / 膜脂質 |
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| Outline of Final Research Achievements |
In C4 plants, membrane structure of mesophyll chloroplasts is susceptible to damage by salinity stress compared with that of bundle sheath chloroplasts. This suggests that there are cell-specific mechanisms of stress response and defense. We have succeeded in isolation of pure mesophyll and bundle sheath cells from salinity-stressed maize, a C4 plant, by using roller and mechanical grinding techniques, and undertook gene expression analysis. In addition, we compared the lipid alterations in mesophyll and bundle sheath chloroplasts in response to salinity stress, and found that the changing pattern of monogalactosyldiacylglycerol could be related to the salt sensitivity of membrane structure.
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| Free Research Field |
作物生理生化学
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