2017 Fiscal Year Final Research Report
Analysis on developmental processes of tannin cells for accumulating proanthocyanidins and their polymers in persimmon fruit
Project/Area Number |
26660026
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Horticultural science
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Research Institution | Ryukoku University (2015-2017) Kyoto University (2014) |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Keywords | 園芸学 / 果樹 / タンニン細胞 / 渋味 |
Outline of Final Research Achievements |
Ovary of persimmon fruit was observed by light microscope at the blooming stage to obtain the developmental process of tannin cells in the fruit, and it revealed that small tannin cells are firstly appeared at the junction of calyx lobe and ovary, and those are distributed toward upper parts of ovary with cell enlargement. At undevelopmental stages of ovary, i.e. 4 or 3 weeks before blooming, tannin cells are only observed in receptacle. When we tested an efficiency of a new fluorescent dye conjugated with fluorescent dye and gelatin to elucidate localization of condensed tannins in persimmon fruit, it showed a successful possibility if preparation method for sectioning was improved. In addition, high gene expression related to proanthocyanidin biosynthesis was detected in the early sage of fruit development in persimmon.
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Free Research Field |
果樹園芸学
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