Development of phytoplasma tolerant plants by a novel immunosuppressive therapy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660032
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The University of Tokyo
• Principal Investigator: Himeno Misako 東京大学, 大学院農学生命科学研究科(農学部), 特別研究員 (10646970)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ファイトプラズマ / 篩部壊死 / 病害防除

Outline of Final Research Achievements

Experimental methods to detect phloem cell death caused by phytoplasma infection were investigated, and the optimized method using trypan blue staining was developed. From the results obtained by LC- TOF-MS analysis, phloem cell death caused by phytoplasma infection was suggested to cause impaired phloem loading, resulting in activating anthocyanin biosynthesis pathway. Further, results of inoculation test using mutant plants suggested that anthocyanin accumulation is involved in the reduction of cell death and plant immune response pathway is associated with diseased symptoms. These results may contribute to development of phytoplasma tolerant plants.

Free Research Field

農学