2016 Fiscal Year Final Research Report
Development of an acaricide resistance gene linkage mapping method
| Project/Area Number |
26660042
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
Thomas Vanleeuwen University of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics, Associate Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ハダニ / ゲノム / 染色体地図 / 薬剤抵抗性 / 遺伝子マーカー |
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| Outline of Final Research Achievements |
Development of a new method analyzing acaricide resistance factor using genome mapping in the two-spotted spider mite was aimed in this study. To construct genome map, genetic linkages among microsatellite (SSR) loci, which position in scaffolds had been determined, were analyzed in the two-spotted spider mite. Consequently, 26 scaffolds of 40 largest scaffolds (> 90% of a hole genome) were mapped into the resulting five linkage groups of SSRs.
Resistance genes to pyridaben, a mitochondrial electron transport complex I inhibitors, was analyzed by QTL analysis. A significant QTL was indicated at scaffold 7 where PSST gene, a component of the complex I, existed, and the significant QTL area was extended to the next 10 cM, where one pseudo and five full-length cytochrome P450 monooxygenase genes were found. Previous studies suggested involvement of pyridaben resistance with cytochrome P450 functions.
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| Free Research Field |
応用昆虫学
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