2015 Fiscal Year Final Research Report
development of a novel screening system for methanorophs producing useful compounds
| Project/Area Number |
26660056
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 発酵生産 / スクリーニング技術 |
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| Outline of Final Research Achievements |
A novel screening system of isolating methanotrophs that produce useful compounds was developed. The facts that methanotrophs cannot use any carbon sources except methane and general bacteria cannot use methane as sole carbon source were the key point of this method. The mixture of assay strains (E. coli, etc) was added in the minimal salt medium and cultured with methane as the sole carbon source. If methanotrophs that produce several organic compounds, assay strains can grow and become clouded around the colony of it.Finally, some methanotrophs that produce organic compound were isolated using this screening system.
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| Free Research Field |
産業微生物資源学
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