Development of a novel method for activating the potential of fungi to produce secondary metabolites by utilizing ribosome-targeting antibiotics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660059
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: HOSAKA Takeshi 信州大学, 学術研究院農学系, 准教授 (50391206)
• Research Collaborator: HAMAUZU Ryoko 信州大学, 農学部・応用分子微生物学研究室, 研究補佐員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 二次代謝 / 糸状菌 / 酵母 / リボソーム攻撃性抗生物質 / 抗生物質耐性変異 / ホルミシス

Outline of Final Research Achievements

Mutations conferring resistance to ribosome-targeting antibiotics and ribosome-targeting antibiotics at subinhibitory concentrations often dramatically alter the phenotypic profile in bacteria. This study has demonstrated that the concept as described above can be applicable to a variety of eukaryotic microorganisms to elicit their potential, such as the ability of fungi Monascus pilosus to produce secondary metabolites，and to alter the phenotypic characteristics of the fission yeast Schizosaccharomyces Japonicus. Interestingly, phenotypic and genetic characterization of the secondary metabolite-overproducing Hygromycin B resistant mutant of M. pilosus revealed that ribosome-targeting antibiotics in eukaryotic microorganisms could have different mechanisms to those in prokaryotic microorganisms for activating their potential.

Free Research Field

応用微生物学