Construction of a novel cytosolic protein expression system based on redox regulation in methylotrophic yeast

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660061
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: Sakai Yasuyoshi 京都大学, (連合)農学研究科(研究院), 教授 (60202082)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: レドックス制御 / タンパク質生産 / メタノール酵母

Outline of Final Research Achievements

Methylotrophic yeasts with strong methanol-inducible promoters have widely been utilized as hosts of heterologous gene expression of useful proteins. However, heterologous gene expression system using methylotrophic yeasts has two problems, highly immunogenicity of yeast glycosylation in human and decrease of expression level of heterologous proteins due to induction of ER stress in. In this study, we have developed the novel expression system in which heterologous proteins are produced in cytosol to avoid yeast glycosylation and ER stress induction and succeeded in expression of heterologous proteins with native activity.

Free Research Field

応用微生物学