Development of Liquid Isolation protocol of microorganisms in a mixed culture system by using fluoro-micromanipulator

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyushu University
• Principal Investigator: Sakai Kenji 九州大学, (連合)農学研究科(研究院), 教授 (50205704)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 蛍光顕微マニピュレーター / 難培養微生物 / 高度好熱菌

Outline of Final Research Achievements

In this study, efficient liquid isolation protocol of not-yet isolated bacteria using fluoro-micromanipulator was developed. Type of cqpilally, plate, and oil as well as position of duple, washing, and application of FDA (reagent for detecting viable cells), observation of micro-colonies were optimized. As a results, two types of interesting microorganisms showing exteme-thermophilicity were successfully purified from high-temperature compost.

Free Research Field

土壌環境微生物学