2015 Fiscal Year Final Research Report
Development of Liquid Isolation protocol of microorganisms in a mixed culture system by using fluoro-micromanipulator
Project/Area Number |
26660069
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
|
Research Institution | Kyushu University |
Principal Investigator |
Sakai Kenji 九州大学, (連合)農学研究科(研究院), 教授 (50205704)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | 蛍光顕微マニピュレーター / 難培養微生物 / 高度好熱菌 |
Outline of Final Research Achievements |
In this study, efficient liquid isolation protocol of not-yet isolated bacteria using fluoro-micromanipulator was developed. Type of cqpilally, plate, and oil as well as position of duple, washing, and application of FDA (reagent for detecting viable cells), observation of micro-colonies were optimized. As a results, two types of interesting microorganisms showing exteme-thermophilicity were successfully purified from high-temperature compost.
|
Free Research Field |
土壌環境微生物学
|