Epigenom analysis of multipotent cells by Lactic Acid Bacteria

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Oita University (2015-2016); Kumamoto University (2014)
• Principal Investigator: Kawano Rie 大分大学, 医学部, 病院特任助教 (20468002)
• Co-Investigator(Renkei-kenkyūsha): OHTA Kunimasa 熊本大学, 生命科学研究部神経分化学講座, 准教授 (90244128) ; NAOFUMI Ito 熊本大学, 生命科学研究部神経分化学講座, 博士研究員
• Research Collaborator: Abdulhaleem F. ; MIHOKO Imori
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 乳酸菌 / リプログラミング

Outline of Final Research Achievements

The lactic acid bacterium induced pluripotent stem cell has ability to differentiate to 3 germ layers (endoblast, mesoderm, ectoderm), like iPS cells or ES cell. We analyzed gene expression using the real-time RT-PCR method when we derived a human fibroblast to multipotent stem cells using a lactic acid bacterium. The lactic acid bacterium induced multipotent stem cell strongly induces the expression of NANOG gene in comparison with iPS cells, ES cells. The NANOG gene product is necessary to maintain the undifferentiated ability of the cell. The possibility that high expression of NANOG gene causes a drop of the increase ability of the lactic acid bacterium induced multipotent stem cell is suggested.
In addition, we are searching among lactic acid bacterium cell body ingredients now what is necessary for undifferentiated ability.

Free Research Field

農芸化学