Molecular mechanism of the VNC indaction of Vibrio parahaemolyticus

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyushu University
• Principal Investigator: Kimura Makoto 九州大学, 農学研究院, 教授 (10204992)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 腸炎ビブリオ / トキシン/アンチトキシン / DNAエンドヌクレアーゼ

Outline of Final Research Achievements

In this study, we examined the inhibitory potency of Vp1843 with Escherichia coli DNA gyrase (Gyr). The result showed that Vp1843, like other ParE toxins, significantly inhibited DNA gyrase, giving rise to a linearized DNA, and that the antitoxin Vp1842 neutralized its DNA gyrase inhibitory activity. Finally, we explored whether vp1842vp1843 is involved in induction into the VNC state by preparing a mutant strain (Δ42/43), in which vp1842/vp1843 in the V. parahaemolyticus genome were knocked out by homologous recombination. The result suggested that vp1842/vp1843 is not involved in the induction into the VNC state. However, we found that the vp1843 expression in E. coli cells strongly induced chromosomal DNA degradation. On the basis of these observations, we suggest that vp1842/vp1843 plays a role in stabilization of the V. parahaemolyticus genome.

Free Research Field

生物化学