THE ESTABLISHMENT OF HIGH-THROUGHPUT ANALYSIS SYSTEM OF MULTIPLE ENZYME ACTIVITIES FOR THE PROMOTION OF NUTRI-OMICS STUDY.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660107
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: The University of Tokyo
• Principal Investigator: Kato Hisanori 東京大学, 総括プロジェクト機構, 教授 (40211164)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 酵素活性 / 一斉分析 / 網羅解析 / 食品 / ハイスループット

Outline of Final Research Achievements

In nutrition study, it is essential to capture the metabolic profile at the level of enzyme activity. However, the measurement of multiple enzyme activity is generally time-consuming and requires a lot of work. Therefore we aimed to establish an easy and rapid method to analyze multiple metabolic enzyme activities using a high-throughput spectrophotometer. Our experiment in rats under feeding/fasting conditions showed that multiple metabolic enzyme activities (at least 16 enzymes) can be measured by rapid and automated method using the high-throughput spectrophotometer. Also, we showed the differential response between the changes of enzyme activity and gene expression.

Free Research Field

分子栄養学