Development of imaging technique for simultaneously visualizing dynamics of multiple dietary factors

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660115
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: Fujimura Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
• Co-Investigator(Kenkyū-buntansha): MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627) ; SETOYAMA Daiki 九州大学病院, 特任助教 (30550850) ; HAYAKAWA Eisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 特任助教 (20739809)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 食品動態解析 / 質量分析イメージング / MALDI-MS / マトリックス

Outline of Final Research Achievements

Herein, we attempted to create the basic label-free technique for simultaneously visualizing multiple food factors within the tissue by improving the drawback of matrix-assisted laser desorption mass spectrometry (MALDI-MS) on the basis of development or original usage of matrix. A brute force MALDI-MS analysis using both matrix library and various kinds of food factors revealed an ionizability and ionization efficacy of food factors and their structure-activity relationship to matrix. In addition, we enabled to obtain information on quality and functionality-related multiple food factors in foodstuff, consisted of various kinds of components and simultaneous visualization of orally dosed multiple food factors within the animal tissue by using newly selected matrix. These results suggest that this matrix screening-based MALDI-MS system may provide an effective strategy for simultaneously detecting quality and functionality-related multiple food factors.

Free Research Field

食品科学