Development of mutation clock using Sasa kurilensis

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660129
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Akita Prefectural University
• Principal Investigator: Inoue Mizuki 秋田県立大学, 生物資源科学部, 助教 (80432342)
• Co-Investigator(Kenkyū-buntansha): MAKITA Akifumi 秋田県立大学, 生物資源科学部, 教授 (60315596) ; SUYAMA Yoshihisa 東北大学, 大学院農学研究科, 准教授 (60282315) ; Suzuki Junichiro 首都大学東京, 理工学研究科, 准教授 (00291237)
• Co-Investigator(Renkei-kenkyūsha): Saito Tomoyuki 独立行政法人森林総合研究所, 森林植生研究領域, 研究員 (00414483)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: NGS / ササ / GBS / SNPs / 齢

Outline of Final Research Achievements

We tried to get genome-wide SNPs using modified GBS and developed new method to estimate the longevity. We used 24 leaves of Sasa kurilensis in Towada, whose longevity has already identified by flowering interval (18years old: each 4 ramets of 2 genet and 8 ramets of 1genet , 80years old: 8 ramets of 1genet). We found no difference in numbers of SNPs among new and old individuals. However, we found the diversity of SNPs was higher in old individuals than in younger ones. It suggested that information from SNPs using NGS might be useful to estimate longevity.

Free Research Field

森林生態学