The development of transparent method of formalin-fixed ovary specimen of fish

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Kyushu University
• Principal Investigator: Matsuyama Michiya 九州大学, (連合)農学研究科(研究院), 教授 (00183955)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 小型浮魚 / 卵巣標本 / バッチ産卵数 / 透明化 / マサバ / マイワシ / マアジ

Outline of Final Research Achievements

The objective of the present study is 1) to develop a method of clearing the formalin-fixed ovary specimens of small pelagic fish such as sardine, horse mackerel, and chub mackerel, and 2) to establish a method to estimate the batch fecundity of the fish using the transparent ovary specimens.
Formalin-fixed ovarian specimens of the three marine fish were treated with SeeDB solution mainly composed of fructose, and we revealed the optimum conditions for conveniently transparent. Further, measuring the number of nuclear migrating eggs in the ovarian samples of jack mackerel enabled to calculate the batch fecundity without using a hydrated eggs.

Free Research Field

魚類生理学