Studies to find a mechanism of gonadal growth stimulation in sea urchin

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660167
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Takagi Yasuaki 北海道大学, 水産科学研究院, 教授 (10212002)
• Co-Investigator(Renkei-kenkyūsha): URA Kazuhiro 北海道大学, 大学院水産科学研究院, 助教 (90360940) ; TODO Takashi 北海道大学, 大学院水産科学研究院, 准教授 (60303111)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ウニ / 生殖巣 / 生殖腺刺激ホルモン / 脂質代謝 / 器官培養 / MYP / ステロイド合成 / ペプチド

Outline of Final Research Achievements

We developed organ culture system using the sea urchin gonads for analysis of endocrine system during the gonadal growth. To date, it is well known that growth of gonad by synthesis and stored of MYP. The aim of this present study is identification of hormonal chemicals to induce the gonadal growth in sea urchin. We performed organ culture using the sea urchin gonads in the coelomic fluids of sea urchin, we measured MYP mRNA levels in the gonads by qPCR after incubation for 3days. The levels of MYP mRNA were no changing after incubation. It is suggested that MYP synthesis by nuclear receptor in gonads. Moreover, we extracted total lipids fraction form the gonads of sea urchin, and then gonads were incubated in the medium containing total lipids. However, NYP mRNA was no induced in this experiment. In the present study, we can’t identify the gonadal stimulation hormone in sea urchin. In future, we will try the comparison of colemic fluids compounds during gonadl growth.

Free Research Field

水産生物生理学