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2016 Fiscal Year Final Research Report

Studies on mechanisms of viral takeover of its cyanobacterial host for application platform of a next generation fermentation system

Research Project

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Project/Area Number 26660171
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionKyoto University

Principal Investigator

Yoshida Takashi  京都大学, 農学研究科, 准教授 (80305490)

Project Period (FY) 2014-04-01 – 2017-03-31
Keywordsシアノファージ / ラン藻 / ミクロキスティス / トランスクリプトーム / 光発酵
Outline of Final Research Achievements

We examined transcriptional dynamics of genes of cyanobacteria Microcystis aeruginosa and its pahge during infection through RNA-seq and in silico analysis. Phage gene dynamics showed three expression classes like other lytic dsDNA phages including marine cyanophages; early (host-takeover), middle (replication) and late genes (virion morphogenesis). The early genes are concentrated in a single ~5.8 kb window on the phage genome spanning 10 open-reading frames. Almost all host genes (99%) did not show significant change in expression throughout infection. Bacterial RNA polymerase (sigma70)-recognition sequences were also found in the upstream region of middle and late genes, but not the phage-specific motifs. Our findings suggested that Ma-LMM01 phage archived sequential three expression patterns with no change in host promoter activity unlike known T4-like phages.

Free Research Field

水圏微生物学

URL: 

Published: 2018-03-22  

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