2015 Fiscal Year Final Research Report
New molecular discrimination for animal species using DNA polymorphism
| Project/Area Number |
26660212
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 分子種判別 / 動物 / ミトコンドリアDNA / DNA鑑定 / 簡便・迅速・正確 / PAS検出 / 共通性と特異性 / 種特異的PCR primer |
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| Outline of Final Research Achievements |
Food problems are various, contamination of the food, such as animal hair, even for such repellent foods such as problems which are allergies, religion and culture, the interest is high not only in Japan but also over the world. In these methods many problems occurred, such as the minute size, poor preservation conditions and absence of DNA for analysis. Here, we used Multiplex PCR that can analyze many samples simultaneously. In addition, printed array strip (PAS) was also used which made analysis quick, simple and cheap. We have designed specific PCR primers in each animal species (domestic, wild and pet animals), and PCR products for all animal species were obtained. So, this method was very useful for species discrimination from fresh, processed meat and food made from domestic and wild animals. Also it was useful for forensics identification of human and animals. Therefore it was possible to construct a fast, simple and high sensitive detection method in molecular discrimination.
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| Free Research Field |
動物遺伝学
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