Function of blood dendritic cells in mastitis cow

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660217
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tohoku University
• Principal Investigator: Aso Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): KITAZAWA HARUKI 東北大学, 大学院農学研究科, 准教授 (10204885) ; NOCHI TOMONORI 東北大学, 大学院農学研究科, 准教授 (10708001) ; WATANABE KOUICHI 東北大学, 大学院農学研究科, 助教 (80261494)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 乳房炎 / 牛 / 樹状細胞 / 枯草菌飼料添加剤 / イムノバイオティック飼料 / CD40 / CD80 / 補助因子

Outline of Final Research Achievements

Dendritic cells (DC) are bone marrow-derived antigen presenting cells. They play an important role in priming adaptive immunity and in mature immune responses against pathogens. The biological activities of DC are highly dependent on their migratory potential and on the expression of adhesion molecules with T lymphocytes. DC secrets the cytokines and then polarizes immune responses to Th1-mediated cellular or Th2-mediated antibody responses.
We obtained blood samples from cows at 30 days before and after parturition, and then purified DC at more than 85% using MACS system. All of DC-fraction cells expressed DC surface makers such as MHC-II, CD172a and CD11c. Probiotic feed containing Bacillus subtilis C-31-2 strain increased the expression of costimulatory molecules CD80/86 and CD40 on the cell surface of DC. These data indicate that probiotics Bacillus subtilis C-31-2 strain is able to modulate the functions of T cells and inhibit the inflammation in mastitis cow.

Free Research Field

細胞生物学