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2015 Fiscal Year Final Research Report

Identification of cellular factors responsible for virus growth by a novel genome editing technology

Research Project

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Project/Area Number 26660227
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

Horimoto Taisuke  東京大学, 農学生命科学研究科, 教授 (00222282)

Project Period (FY) 2014-04-01 – 2016-03-31
Keywordsウイルス / CRISPR/Cas9
Outline of Final Research Achievements

Cellular factors are essential for viral growth. To identify these factors, we used a novel genome editing CRISPR/Cas9 system in combination of viral vectors. We constructed a retroviral vector library expressing random targeting gRNA/Cas9 and infected cell culture with the library. Then, following infection of influenza or Akabane virus, we selected cells resistant to virus infections. Through these procedures, we identified heparan sulfate proteoglycan as an cellular attachment factor for Akabane virus.

Free Research Field

獣医ウイルス学

URL: 

Published: 2017-05-10  

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