Studies on the function of the long noncoding region in herpesvirus replication

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660228
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ヘルペスウイルス / ロングノンコーディングRNA / 遺伝子発現調節

Outline of Final Research Achievements

Virus genes are efficiently arranged on the genome. However, the herpesvirus genomes have long non-coding regions on their genomes. The purpose of the present study is to reveal functions of the long non-coding regions in viral replication using equine herpesvirus 1 (EHV-1) as a model virus. Two long non-coding RNA, 1028 bps (lncRNA-X1) and 679 bps (lncRNA-X2), were found as transcripts. It was found that several gene expressions were under detection limits in murine cultivated neuronal cell infected by a mutant EHV-1 lacking lncRNA-X1 and lncRNA-X2. The results indicated that the lncRNAs might have roles for EHV-1 replication in neuronal cells.

Free Research Field

獣医ウイルス学