The preparation of gene knock out tick by TALEN using nuclease for infectious model

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660229
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: TANAKA TETSUYA 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (00322842)
• Co-Investigator(Renkei-kenkyūsha): MOCHIZUKI MASAMI 鹿児島大学, 農水産獣医学域獣医学系, 教授 (90157834)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マダニ / マダニ細胞 / アクチンプロモータ－ / フェリチンプロモーター / CAGプロモーター / PGKプロモーター

Outline of Final Research Achievements

Although tick studies were conducted by gene silencing using RNA interferance for analyzing the gene function of tick, the skills of gene knockeout and gene transgenic for ticks have not been developing at the morment.Therefore, we tried to incorporate foreign gene into the tick cell line for establishing gene enginering. Although the effect of gene plasmid transfeciton into the tick cell line showed high luceferase activity using pmiGLO or pCAGGS-Luceferase,the effect of plasmid transfection into the tick cell line showed low luceferase activity using actin or ferritin pomotor.These studies indicated that the foreign gene incorporated into the tick may make a break through the praparation of gene konockout or trangenic tick.

Free Research Field

節足動物分子工学