Research for novel canine tumor markers using tissue microarray

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660236
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: Chambers James 東京大学, 農学生命科学研究科, 助教 (00621682)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Hiroyuki 東京大学, 大学院農学生命科学研究科, 教授 (40155891) ; UCHIDA Kazuyuki 東京大学, 大学院農学生命科学研究科, 准教授 (10223554)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 獣医学 / 組織マイクロアレイ / イヌ / 腫瘍マーカー

Outline of Final Research Achievements

Protein expression in normal canine tissues were examined by using tissue microarray. We next searched for diagnostic markers of various tumors including histiocytic tumors and intestinal polyps. For histiocytic tumors, CD204 and CD163 were found to be reliable diagnostic markers, furthermore expression levels of these markers differed among the site of occurrence of the tumor. In respect of intestinal polyps, we investigated intestinal polyps of Miniature Dachshunds and Jack Russel Terriers. In the early stage, highly differentiated epithelial cells expressed CD20; in the late stage, foci of atypical epithelial cells were negative for CD20 and the nucleus of these cells were positive for β-catenin. These results suggests malignant transformation in canine intestinal polyps.

Free Research Field

獣医病理学