Development of a new diagnostic procedure for bovine leukemia virus infection using bovine milk exosomes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660239
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Inoshima Yasuo 岐阜大学, 応用生物科学部, 准教授 (20355184)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: エキソソーム / 生乳 / バルク乳 / 牛白血病 / 牛白血病ウイルス / 診断法

Outline of Final Research Achievements

We tried to establish a method for efficient isolation and purification of exosomes from bovine raw milk and to develop a new diagnostic procedure for bovine leukemia virus (BLV) infection using milk exosomes. Our study revealed that commercially available exosome-isolation kits are applicable to isolate bovine milk exosomes after removal of milk casein by sequential ultracentrifugation. BLV antigens and DNA were not detected from milk exosomes from BLV-infected young cattle subclinically. These results suggest that detectability of BLV antigens and DNA in milk exosomes are conceivably related to the copy numbers in cattle.

Free Research Field

動物感染症学