Establishment of detection system for canine circulating tumor cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 26660243
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: BONKOBARA Makoto 日本獣医生命科学大学, 獣医学部, 准教授 (50343611)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: 血中循環腫瘍細胞 / 犬

Outline of Final Research Achievements

Detection and counting of the circulating tumor cells (CTC) could be one of the potential approaches to predict early metastasis of cancer cells. In the present study, we therefore attempted to establish FACS-based CTC counting system using canine peripheral bold spiked with various number of model cancer cells (MDCK cells); however, the counting accuracy was not sufficient for evaluation of the number of CTC due to white bold contaminations. We then examined quantification of CTC using quantitative PCR method. Among epithelial cell-associated 35 molecules examined, CK19, AGR2, IRX3, UPKB1, and TACC2 were detected by this method only in blood spiked with MDCK cells. Particularly, CK19 and TACC2 were detected at the minimum concentration of 1 MDCK cell/1 ml blood. From these findings, CK19 and TACC2 could be potential markers for detecting CTC by quantitative PCR method.

Free Research Field

獣医臨床病理学