2016 Fiscal Year Final Research Report
Development of novel technology for prevention of mastitis using nanotechnology
| Project/Area Number |
26660245
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 黄色ブドウ球菌 / 乳房炎 / 抗体 |
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| Outline of Final Research Achievements |
Staphylococcus aureus is a major pathogen that occasionally causes subclinical mastitis associated with huge economic losses to the dairy industry. We generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cell surface polysaccharides, or both, may trigger the inhibition of bacterial growth by anti-S. aureus. Together, our findings contribute insights into developing new strategies to further improve the available mastitis vaccine by designing a novel antigen on the surface of S. aureus to induce inhibitory signals that inhibit bacterial growth.
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| Free Research Field |
動物免疫学、動物微生物学
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