Assesment of cellular quality using methylated DNA reporter mouse, MethylRO

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660250
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kinki University (2015); Osaka University (2014)
• Principal Investigator: YAMAGATA Kazuo 近畿大学, 生物理工学部, 准教授 (10361312)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ＤＮＡメチル化 / 細胞評価 / メチロー / ライブセルイメージング

Outline of Final Research Achievements

In 2014-2015, we elucidated that intensity and intra-nucleic pattern of methylated DNA were dynamically changed during cellular differentiation by using ES cells and preimplantation embryos. With these data, we have reported and published in Stem Cell Report Journal. In 2015-2016, by improving the above "long-term and high-resolution live-cell imaging technology", we determined the experimental conditions to capture and quantify the processes of colony formation of ES cells from single cell and of germ cell formation from ES cell.

Free Research Field

発生工学、生殖生物学