2016 Fiscal Year Final Research Report
Studies on the freeze-drying of mammalian preimplantataion embryos
Project/Area Number |
26660261
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Integrative animal science
|
Research Institution | Kindai University |
Principal Investigator |
KATO Yoko 近畿大学, 農学部, 教授 (40278742)
|
Co-Investigator(Renkei-kenkyūsha) |
TANI Tetsuya 近畿大学, 農学部, 講師 (70319763)
|
Research Collaborator |
TAGUCHI Misato 近畿大学, 大学院・農学研究科
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 凍結乾燥 / 初期胚 / 哺乳動物 |
Outline of Final Research Achievements |
In this study, we examined whether pre-implantation stage embryos in mammals could be freeze-dried like sperm and somatic cells. When mouse blastocysts were freeze-dried, trehalose supplemented medium for freeze-drying and isotonic solution for rehydration were effective for their blastocoele recovery. For porcine blastocysts, no blastocysts recovered their blastocoele after rehydration in any approach.
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Free Research Field |
動物発生工学
|