2016 Fiscal Year Final Research Report
Purification and identification of a novel PTTH receptor in Bombyx mori
| Project/Area Number |
26660266
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Aichi Gakuin University (2015-2016)
Nagoya University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 昆虫 / 前胸腺刺激ホルモン / 受容体 / カイコガ |
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| Outline of Final Research Achievements |
A putative PTTH receptor on the plasma membrane of the silkmoth prothoracic glands (PGs) was detected by Western blotting. The conjugate of this protein with PTTH had Mr of 120kDa and was not detected in any tissues other than the PGs. To purify this protein, the PGs were incubated with His-tagged PTTH, and the PTTH and receptor were linked to each other with a cross-linker, followed by solubilization with detergent. The resultant conjugate was successfully affinity-purified using magnetic beads for His-tag isolation, although the purity was still insufficient for sequence analysis. We tried to use immunoprecipitation for further purifying the PTTH-receptor conjugate without success.
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| Free Research Field |
昆虫内分泌学
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