2015 Fiscal Year Final Research Report
Dying marker of host insects which induces the emergence of parasitoid wasp larvae
| Project/Area Number |
26660272
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Saga University |
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Principal Investigator |
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| Research Collaborator |
MATSUMOTO Hitoshi
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 昆虫 / 寄生蜂 / 脱出誘導 / 生理活性因子 |
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| Outline of Final Research Achievements |
Female wasps of parasitoid Cotesia kariyai oviposit several tens of eggs into the host larvae of armyworm Pseudaletia separata at each parasitization and the wasp larvae emerge from their hosts almost simultaneously about 10 days after parasitization. We found that wasp larvae initiated their emergence from the posterior part of the host larvae tightly ligated at arround the middle of their abdomen shortly before the wasp emergence. Since the wasp emergence was found to be induced by a host hemolymph factor, we performed the purification and characterization of the factor. The structure of the purified active factor was determined by LC-MS/MS and NMR analyses. Furthermore, we confirmed that the active factor was secreted from hemocytes. The results of a series of incubation experiments suggested that the secretion of the active factor from hemocytes was induced by a novel factor secreted from brain-9th abdominal ganglion.
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| Free Research Field |
昆虫生化学
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