2016 Fiscal Year Final Research Report
Preparation for PTD-Pin1 and its appllication to stem cell differentiation
| Project/Area Number |
26660287
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Pin1 / 間葉系幹細胞 / 細胞分化 / 細胞膜透過性Pin1 |
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| Outline of Final Research Achievements |
We prepared for (RGD)2-TAT-Pin1 as an novel Protein transduction domain (PTD)-Pin1 consisting of 2 of Arg-Gly-Asp（RGD）motif that binds to the integrin α5β1 expressing on mesenchymal stem cell membranes at the N-terminal end, trans-activator of transcription (TAT）and Pin1. Function of Pin1 was evaluated with the Pin1-knockout ASC clone prepared from the Pin1-/-；p53-/- mouse. The differentiation of ASCs into adipocytes was significantly improved by (RGD)2-TAT-Pin1. The effect of (RGD)2-TAT-Pin1 on differeantiation of ASC into osteoblasts and chondrocytes are being studied.
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| Free Research Field |
応用分子細胞生物学
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