Role of microRNA on innate immune responses against intracellular microbe

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Yano Tamaki 東北大学, 薬学研究科(研究院), 准教授 (50396446)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 自然免疫 / 細胞内感染 / miRNA / ウイルス感染

Outline of Final Research Achievements

For analyzing the function of microRNA in the regulation of innate immune responses against intracellular microbes, such as intracellular bacteria or viruses, we performed next generation sequencing of miRNA in Drosophila. As a result, we identified 5 miRNA and 2 miRNA, expression of which is increased to Listeria or Vesicular stomatitis virus infection, respectively. miR-14, present in common of the both above, functions in autophagic cell death. We further analyzed the fuction of Ref(2)P, an adaptor of the selective autophagy, to show that it is essential against virus infection, and when viruses are orally infected to Drosophila adults, Ref(2)P makes an aggregate in intestinal epithelial cells. These results suggest the importance of Ref(2)P aggregate formation in the innate immune responses against viruses, and its regulation could be a novel target of virus regulation.

Free Research Field

細胞生物学