Transmission of GPCR signals among cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670024
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: HATTORI Mitsuru 東京大学, 理学(系)研究科(研究院), 研究員 (20589858)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: GPCR / ルシフェラーゼ / 細胞

Outline of Final Research Achievements

G protein coupled receptors (GPCRs) are one of the remarkable targets for the design of therapeutic drugs. Many of screening methods have been developed to identify effective agents for GPCR signaling. We previously developed a bioluminescence measuring system to detect the interaction of GPCR with β-arrestin based on split luciferase fragment complementation. During the observation of bioluminescence, there is one of the hypotheses that GPCR signals transmit among cells.
To demonstrate the hypothesis, we developed new bioluminescence imaging technique for the temporal monitoring of GPCR-β-arrestin interaction in living cells. Simultaneous imaging of GPCR activity and cell movements indicated that GPCR activity linked the stage of cell proliferation.

Free Research Field

分子生物学