investigation using NMR for molecular targets and stereochemistry of marine macrolides

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670045
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Kochi University
• Principal Investigator: TSUDA Masashi 高知大学, 教育研究部総合科学系, 教授 (10261322)
• Co-Investigator(Kenkyū-buntansha): OGURA Kenji 石川県立大学, 生物資源環境学部, 教授 (50270682) ; ULANOVA Dana 高知大学, 教育研究部総合科学系, 助教 (70610129)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: マクロリド / 殺細胞活性 / 細胞増殖促進活性 / 渦鞭毛藻

Outline of Final Research Achievements

Marine dinoflagellates of the genus Amphidinium have been recognized as a valuable source of unique macrolides possessing exhibiting potently cytotoxic activity. In this study, the investigation for molecular targets of the cytotoxic macrolide, iriomoteolide-1a, resulted in affinity for tubulin. Furthermore, we isolated two new compounds, amphirionin-4 with potent cell proliferation-promoting activity and iriomoteolide-2a, a 23-membered macrolide with cytotoxic activity, and their structures with absolute stereochemistries were elucidated.

Free Research Field

薬学