2015 Fiscal Year Final Research Report
Exhaustive analyses of mechanism of plant cell death using fluorobody
| Project/Area Number |
26670046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hiroyuki 九州大学, 薬学研究院, 准教授 (30253470)
SAKAMOTO Seiichi 九州大学, 薬学研究院, 助教 (50610177)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 細胞死 / モノクローナル抗体 / ｓｃFV / fluorobody / cytochrome c / cyclophilin D / VDAC |
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| Outline of Final Research Achievements |
I cloned and expressed genes encoding three factors (cyclophilin D, CycD; voltage-dependent anion channel, VDAC; cytochrome c, Cytc), which are participated with plant cell death. To prepare the monoclonal antibodies against these factors, I injected these recombinant proteins. As a result, I could obtain the monoclonal antibodies, which highly specifically bind to each antigen. Next, I constructed the gene encoding scFV against these factors, and succeeded in the expression of these genes in E. coli.
CycD gene, which was cloned in this study, was found to be not involved in cell death, because the recombinant CycD did not bind to cyclosporine A. Therefore, I attempt to clone the genes encoding the CycD actually mediating cell death in plant and to prepare monoclonal antibody against this CycD. Now I attempt to construct fluorobody genes by connecting scFV genes with green-fluorescent-protein gene, and develop genetically engineered plant by introducing the fluorobody.
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| Free Research Field |
生薬学
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