2016 Fiscal Year Final Research Report
Study on the regioselective coupling reaction to produce atropisomers by enzyme
| Project/Area Number |
26670050
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 二量体化 / マルチカッパーオキシダーゼ / ビアリルカップリングエンザイム / ダイナピノンA |
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| Outline of Final Research Achievements |
Atropisomer dinapinones A1 and A2 (DPA1 and DPA2) were isolated from a culture of Talaromyces pinophilus FKI-3864. Monapinone coupling enzyme (MCE) which dimerizes naphthopyranone monapinone A (MPA) to regioselectively synthesize DPA (a mixture of atropisomers DPA1 and DPA2) was purified from T. pinophilus FKI-3864. In this study, we established an efficient procedure to purify MCE easily and heterologous expression system of MCE gene in Escherichia coli, Aspergilluys oryzae and Saccharomyces cervisiae. Furthermore, to identify regioselective coupling reaction of MCE, enzymatic analysis and substrate specificity were investigated.
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| Free Research Field |
天然物化学・生化学
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