Development of gene delivery system using the tissue press/suction method

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670082
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Nagasaki University
• Principal Investigator: KAWAKAMI Shigeru 長崎大学, 医歯薬学総合研究科(薬学系), 教授 (20322307)
• Co-Investigator(Renkei-kenkyūsha): WADA Mitsuhiro 九州保健福祉大学, 薬学部, 教授 (40295093)
• Research Collaborator: HAGIMORI Masayori ; FUMOTO Shintaro ; FUCHIGAMI Yuki ; OYAMA Natsuko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 遺伝子 / 医療・福祉 / バイオテクノロジー / バイオ関連機器 / 遺伝子治療 / 組織押圧法 / 組織吸引圧法

Outline of Final Research Achievements

We previously developed tissue press/suction method as a selective gene delivery method. In this study, we developed sustained gene delivery system using the tissue suction method and evaluated the efficiency of transgene expression in pathological model mice to develop novel gene delivery strategy with tissue suction method or apply to analysis of gene function.
For sustained transgene expression, pCpGfree-Lucia was used and expressed for 2 weeks both in the kidney and the liver. We also studied the distribution of transgene expression using tissue clearing method to construct treatment strategy. As a result, transgene the kidney suction method delivered was observed in interstitial area around blood vessel. In the liver suction method, transgene was observed in suctioned site and mainly in a superior part of the liver. In addition, characteristics of transgene expression both in carbon tetrachloride induced acute hepatitis model mice and hepatic fibrosis model mice were clarified.

Free Research Field

医療系薬学