Comprehensive screening of histone modifying enzyme

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670085
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Keiko 東北大学, 医学(系)研究科(研究院), 教授 (60294972)
• Co-Investigator(Kenkyū-buntansha): FUNAYAMA Ryo 東北大学, 大学院医学系研究科, 助教 (20452295)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: CRISPR/Cas9 / メチル化酵素 / ES細胞

Outline of Final Research Achievements

In the nucleus, core histones, which genomic DNA wrap around, create an environment of genome DNA. Post-translational modifications of histone are thought to regulate the shape of existence and function of genomic DNA. This means the significance to elucidate the regulatory mechanisms of post-translational modifications of histone.
In this project, the final goal was set as the generation of cell group which is consist of cells with mutation of various enzymes of modification, and the examination of change of constituent cells after long-term culture or induction of differentiation. Effective protocol of introduction of mutation was determined for several cell lines.

Free Research Field

分子生物学