A novel screening method to identify any defined RNAi combinations to induce cell reprogramming.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670088
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Katoh Hiroto 東京医科歯科大学, 難治疾患研究所, 助教 (60446549)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 細胞分化 / 次世代シーケンス / shRNAライブラリ

Outline of Final Research Achievements

The aim of this study was to develop fundamental research protocols for a novel high-throughput screening method in order to identify any defined RNAi combinations to control cell lineages via novel RNAi-based reprograming strategies. Particularly, to develop such a screening method, I combined whole-genomic shRNA lentivirus library and next-generation sequence technique. I developed and fine-tuned several complexed experimental protocols related to Lentivirus production, Lentivirus infection, cell culture, cell-sorting or cell-picking, and next-generation sequencing. I could establish some of the fundamental research protocols to be utilized in the high-throughput screening to search for defined RNAi combinations to induce lineage reprograming.

Free Research Field

ゲノム病理学