Functional analysis of microRNA in gonadogenesis and gonadal function.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670098
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: National Center for Child Health and Development
• Principal Investigator: TAKADA Shuji 国立研究開発法人国立成育医療研究センター, システム発生・再生医学研究部, 部長 (20382856)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マイクロRNA / 生殖腺 / マウス

Outline of Final Research Achievements

Roles of microRNA in gonadogenesis and gonadal function were analyzed. Gonad specific knockout mouse of Dicer, that is essential for microRNA biogenesis, was designed based on genome editing. The mouse was generated as designed, but Dicer seems not to be knocked out. A transgenic mouse containing a forced expression cassette of a target gene of microRNA-202, which is expressed specifically in gonads, was generated, but the phenotype was indistinguishable to that of wild type mouse. Ovaries of F2 generation of microRNA-202 KO mouse were more luteinized than those of wild type, implying that some of microRNA have a role in ovarian function.

Free Research Field

発生遺伝学