Live-imaging analysis of vesicle trafficking dynamics in isolated skeletal muscle fiber

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tohoku University
• Principal Investigator: Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 細胞内小胞輸送 / ライブイメージング / 骨格筋 / GLUT4

Outline of Final Research Achievements

This study attempted to develop a novel approach for quantifying intracellular vesicle trafficking dynamics utilizing Quantum-dot-based single molecule imaging in a isolated skeletal muscle fiber from mouse. Intracellular dynamics of GLUT4, an insulin-responsive glucose transporter, as well as of transferrin receptor (TfR) were successfully quantified with this novel approach. In addition, the superresolution G-STED microscopy was employed to observe intracellular vesicles and organelles containing GLUT4 and/or TfR.

Free Research Field

分子糖尿病学