2015 Fiscal Year Final Research Report
Live-imaging analysis of vesicle trafficking dynamics in isolated skeletal muscle fiber
Project/Area Number |
26670099
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General physiology
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Research Institution | Tohoku University |
Principal Investigator |
Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 細胞内小胞輸送 / ライブイメージング / 骨格筋 / GLUT4 |
Outline of Final Research Achievements |
This study attempted to develop a novel approach for quantifying intracellular vesicle trafficking dynamics utilizing Quantum-dot-based single molecule imaging in a isolated skeletal muscle fiber from mouse. Intracellular dynamics of GLUT4, an insulin-responsive glucose transporter, as well as of transferrin receptor (TfR) were successfully quantified with this novel approach. In addition, the superresolution G-STED microscopy was employed to observe intracellular vesicles and organelles containing GLUT4 and/or TfR.
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Free Research Field |
分子糖尿病学
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