Development of the measuring technique of ATP dynamics in beating whole heart

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670100
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Kyoto University (2015); Gunma University (2014)
• Principal Investigator: yamamoto masamichi 京都大学, 医学(系)研究科(研究院), 特任講師 (70423150)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アデノシン三リン酸

Outline of Final Research Achievements

In 2009, it was reported that the distribution of ATP dynamics in culture cell by ATeam, which was developed for FRET probe detecting ATP levels in vivo. In this study, I tried to develop novel ATeam which measure the correct ATP levels for high resolution and high speed, then was successful in developing the powerful mouse measuring the ATP dynamics in mouse. I tried to examine the ATP dynamics in beating heart under the fluorescent microscope. At the result, it was clear that ATP levels are dependent on beating in heart.

Free Research Field

代謝イメージング