転写環境を再構成したDNAビーズによる転写制御因子同定法の確立

2015 Fiscal Year Annual Research Report

• Project/Area Number: 26670130
• Research Institution: Hokkaido University
• Principal Investigator: 高橋 秀尚 北海道大学, 医学(系)研究科(研究院), 講師 (30423544)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 発現制御 / 転写

Outline of Annual Research Achievements

近年、ES細胞の多能性を規定する因子として発見された4つの因子が、全て転写因子であったことから、転写因子による遺伝子発現制御の重要性が再認識されている。本研究では転写因子による遺伝子発現制御の解明を目的に、さまざまな転写因子によっての転写調節領域に特異的にリクルートされる転写のコアクチベーターやクロマチン制御因子を網羅的に同定する方法の確立を行った。本研究遂行によって、転写制御因子の1つメディエーター複合体が、サブユニットMed26を介して、2つの異なる転写伸長因子複合体Super elongation complex（SEC）とLittle elongation complex（LEC）を、それぞれ異なるタイプの遺伝子領域にリクルートすることが明らかとなった。【Takahashi H, et. al. Nature communications 2015】。Med26がどのようなメカニズムによって、2つの異なる転写伸長因子複合体を異なる遺伝子領域にリクルートするのかについて明らかにすることが今後の課題となる。

Research Products

• [Int'l Joint Research] Stowers Institute for Medical Research(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Stowers Institute for Medical Research
• [Journal Article] Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2. (2016)
  • Author(s): Hayashi M, Maehara K, Harada A, Semba Y, Kudo K, Takahashi H, Oki S, Meno C, Ichiyanagi K, Akashi K, Ohkawa Y. et al.
  • Journal Title: Journal of Cellular Biochemistry Volume: 117(3) Pages: 780-792
  • DOI: 10.1002/jcb.25368.
  • Peer Reviewed
• [Journal Article] TRIM39 negatively regulates the NFkB-mediated signaling pathway through stabilization of cactin. (2016)
  • Author(s): Suzuki M., Watanabe M., Nakamaru Y., Takagi D., Takahashi H., Fukuda S. and Hatakeyama S. et al.
  • Journal Title: Cellular and Molecular Life Sciences Volume: 73 Pages: 1085-1101
  • DOI: 10.1007/s00018-015-2040-x
  • Peer Reviewed
• [Journal Article] Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes. (2016)
  • Author(s): Fujieda Y., Amengual O., Matsumoto M., Kuroki K., Takahashi H., Kono M., Kurita Y., Otomo K., Kato M., Oku K., Bohgaki T., Horita T., Yasuda S., Maenaka K., Hatakeyama S., Nakayama K.I. and Atsumi T. et al.
  • Journal Title: Rheumatology Volume: In press Pages: In press
  • Peer Reviewed
• [Journal Article] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex. (2015)
  • Author(s): Takahashi H, Takigawa I, Watanabe M, Anwar D, Shibata M, Tomomori-Sato C, Sato S, Ranjan A, Seidel CW, Tsukiyama T, Mizushima W, Hayashi M, Ohkawa Y, Conaway JW, Conaway RC, *Hatakeyama S.
  • Journal Title: Nature communications Volume: 5:5941 Pages: 5:5941
  • DOI: 10.1038/ncomms6941
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling. (2015)
  • Author(s): Tsukiyama T, Fukui A, Terai S, Fujioka Y, Shinada K, Takahashi H, Terry P. Yamaguchi, Ohba Y, and *Hatakeyama S. et al
  • Journal Title: Molecular and Cellular Biology Volume: 35(11) Pages: 2007-2023
  • DOI: 10.1128/MCB.00159-15
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ. (2015)
  • Author(s): Watanabe M, Takahashi H, Saeki Y, Ozaki T, Itoh S, Suzuki M, Mizushima W, Tanaka K, *Hatakeyama S. et al.
  • Journal Title: Elife Volume: 23(4) Pages: 23(4)
  • DOI: 10.7554/eLife.05615
  • Peer Reviewed / Open Access
• [Journal Article] TRIM29 regulates the p63-mediated pathway in cervical cancer cells. (2015)
  • Author(s): Masuda, Y., Takahashi H, and *Hatakeyama, S. et al.
  • Journal Title: Biochimica et Biophysica Acta Molecular Cell Research Volume: 1853 Pages: 2296-2305
  • DOI: 10.1016/j.bbamcr.2015.05.035
  • Peer Reviewed
• [Journal Article] TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. (2015)
  • Author(s): Masuda Y, Takahashi H, Sato S, Tomomori-Sato C, Saraf A, Washburn MP, Florens L, Conaway RC, Conaway JW, *Hatakeyama S. et al.
  • Journal Title: Nature communications Volume: 6:7299 Pages: 6:7299
  • DOI: 10.1038/ncomms8299
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Role of Human Mediator Subunit Med26 in Transcription Elongation (2015)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアホテル（兵庫県、神戸市）
  • Year and Date: 2015-12-01 – 2015-12-04
  • Invited
• [Presentation] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex (2015)
  • Author(s): Hidehisa Takahashi, Ichigaku Takigawa, Masashi Watanabe, Delnur Anwar, Mio Shibata, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Chris W. Seidel, Tadasuke Tsukiyama, Wataru Mizushima, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama.
  • Organizer: Mechanism of Eukaryotic Transcription
  • Place of Presentation: Cold spring harbor laboratory（NY, USA）
  • Year and Date: 2015-08-25 – 2015-08-29
  • Int'l Joint Research
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2015)
  • Author(s): 高橋秀尚, 畠山鎮次
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: ホテルライフォート札幌（北海道札幌市中央区）
  • Year and Date: 2015-07-15 – 2015-07-17
  • Invited