2015 Fiscal Year Final Research Report
Study on regulation of addipocyte differentiation through the control of mRNA splicing activity
| Project/Area Number |
26670134
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
Kaida Daisuke 富山大学, 先端ライフサイエンス拠点, 准教授 (60415122)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | スプライシング / snRNP |
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| Outline of Final Research Achievements |
We treated mouse 3T3-L1 cells with a splicing inhibitor that binds and inhibits U2 snRNP, and found that adipogenesis was inhibited in a dose dependent manner. The significant inhibition of adipogenesis was observed if the cells were treated with the splicing inhibitor when the cells were cultured in differentiation medium.
We also found that some introns showed resistance against splicing inhibition in a intron- and snRNA-dependent manner. These dependency might affect differentiation efficiency.
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| Free Research Field |
分子生物学
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