2016 Fiscal Year Final Research Report
Gene therapy using the in vivo gene targeting technology
| Project/Area Number |
26670140
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Matsuda Takahiko 京都大学, 物質－細胞統合システム拠点, 研究員 (40313093)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ゲノム編集 / 網膜 / 内在性蛋白質 |
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| Outline of Final Research Achievements |
By combining the CRISPR/Cas9 genome editing technology with an electroporation technique, we succeeded in creating GFP (RFP) knock-in alleles, from which GFP (RFP)-tagged endogenous proteins are produced, in neurons and glial cells in vivo in the postnatal mouse retina.
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| Free Research Field |
分子生物学
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