Study on the fluctuation of ectodomain shedding and its application to predict cancer cell dynamics

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670143
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Ehime University
• Principal Investigator: HIGASHIYAMA SHIGEKI 愛媛大学, プロテサイエンスセンター, 教授 (60202272)
• Co-Investigator(Renkei-kenkyūsha): NANBA DAISUKE 東京医科歯科大学, 難治疾患研究所, 准教授 (10380255) ; FUKUDA SHINJI 愛媛大学, プロテオサイエンスセンター, 講師 (70398238) ; NAKAYAMA HIROINAO 愛媛大学, プロテオサイエンスセンター, 助教 (40512132)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: エクトドメイン・シェディング / がん細胞不均一性

Outline of Final Research Achievements

We analyzed the relation between phenotypic transition of tumor cells and ectodomain shedding activity of membrane-anchored growth factor proHB-EGF in breast tumor MCF-7 cells. We established two MCF-7 clones and further analyzed. One (clone #8) mostly generated epithelial-like colonies, while the other (clone #5) produced both epithelial- and fibroblast-like colonies. We demonstrated that　 proHB-EGF was more shed in breast tumor MCF-7 cells with epithelial-like morphology than those with fibroblast-like morphology. We speculate that ectodomain shedding of proHB-EGF could be associated with morphological phenotype transition of breast tumor cells.

Free Research Field

医科学一般