2014 Fiscal Year Final Research Report
Regulation of cancer malignancy through the cargo sorting into exosomes
| Project/Area Number |
26670158
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Taro 神戸大学, 大学院医学研究科, 准教授 (80304088)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Keywords | エキソソーム / S1P / スフィンゴシン・キナーゼ / Met |
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| Outline of Final Research Achievements |
In the present studies we investigated the role of S1P signal in the cargo sorting into exosomes. As for the cargo molecule, we focused Met protein, which is known to play roles in the malignancy of several cancers. We cultured human melanoma B16-F10 cells in the absence or presence of sphingosine kinase (SK) inhibitor, HACPT for 1 day and quantitated the amount of Met in the exosomes. HACPT caused a significant decrease in Met amount compared with the control. However, knockdown of SK2 by a specific siRNA showed no significant changes in the amount of Met. We are now preparing SK2-nockout cells by a CRISPR/Cas system to investigate further the role of S1P signal in the Met sorting into exosomes.
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| Free Research Field |
生化学
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