2015 Fiscal Year Research-status Report
自然癌化モデルを用いた癌化に関与する特異的な代謝機構の解明と治療標的の探索
| Project/Area Number |
26670163
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| Research Institution | Keio University |
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Principal Investigator |
南嶋 洋司 慶應義塾大学, 医学部, 講師 (20593966)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 老化 / 癌化 / 細胞周期 / 代謝 |
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| Outline of Annual Research Achievements |
初年度には細胞の老化→自然癌化モデルを作製した。
本年度は、増殖期 (Phase I)、増殖期の正常細胞を接触阻害によってG0期に静止させたもの(Phase II)、老化期 (Phase III) 癌化後(Phase IV)につき、樹立した細胞株全てに於いてそれぞれ安定同位体13Cで標識したグルコースを投与し、13Cを含む代謝産物の網羅的定量解析を行った。
その結果、老化細胞特異的、癌か細胞特異的に上昇している代謝産物や代謝経路を同定することが出来た。
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
当初の予定通り進行している。
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| Strategy for Future Research Activity |
最終年度は同定した代謝経路を人為的に操作した細胞株で老化や癌化の誘導や回避が出来るかどうかを検証する。
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[Journal Article] Inhibition of the Oxygen Sensor PHD2 in the Liver Improves Survival in Lactic Acidosis by Activating the Cori Cycle2015
Author(s)
Suhara T, Hishiki T, Kasahara M, Hayakawa N, Oyaizu T, Nakanishi T, Kubo A, Morisaki H, Kaelin WG Jr., Suematsu M, Minamishima YA§. (§Corresponding author)
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 112
Pages: 11642-7
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Glycosaminoglycan as an energy source for pancreatic cancer cells.2016
Author(s)
Shotaro Kishimoto*, Yoji Andrew Minamishima*, Noriyo Hayakawa and Makoto Suematsu (*Equally contributed)
Organizer
10th AACR-JCA Joint Conference on Breakthroughs in Cancer Research: From Biology to Therapeutics.
Place of Presentation
Hyatt Regency Maui (Maui, Hawaii, U.S.A.)
Year and Date
2016-02-18 – 2016-02-18
Int'l Joint Research
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[Presentation] Inhibition of the oxygen sensor PHD2 in the liver improves survival in lactic acidosis by activating the Cori cycle2015
Author(s)
Tomohiro Suhara*, Takako Hishiki, Masataka Kasahara, Noriyo Hayakawa, Tomoko Oyaizu, Tsuyoshi Nakanishi, Akiko Kubo, Hiroshi Morisaki, William G. Kaelin Jr., Makoto Suematsu, Yoji Andrew Minamishima
Organizer
第3回低酸素研究会
Place of Presentation
早稲田大学先端生命医科学センター （東京都新宿区）
Year and Date
2015-07-25 – 2015-07-25
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[Presentation] Inactivating HIF-prolyl hydroxylase PHD2 in liver improves survival after lactic acidosis by activating Cori cycle2015
Author(s)
Tomohiro Suhara, Takako Hishiki, Masataka Kasahara, Noriyo Hayakawa, Tomoko Oyaizu, Tsuyoshi Nakanishi, Akiko Kubo, Hiroshi Morisaki, Makoto Suematsu, Yoji Andrew Minamishima*
Organizer
Keystone Symposia “Hypoxia: From Basic Mechanisms to Therapeutics (E3)”.
Place of Presentation
Royal Dublin Society (Dublin, Ireland)
Year and Date
2015-05-14 – 2015-05-14
Int'l Joint Research
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